Abstract: . . .  tetracycline-inducible bacterial expression Constitutive Baculovirus Expression Vector pLP-BacPAK9 Constitutive baculoviral expression construct pLP-BacPAK9-6xHN Constitutive baculoviral expression construct with 6xHN tags Creator™ Donor Vector or your vector Cm r SacB loxP loxP Gene Page 5 Clontech Laboratories, Inc. 1290 Terra Bella Ave. Mountain . . .
. . .  Amount of vector in the BD In-Fusion Reaction (ng) 25 50 100 200 Avg. no. of resulting colonies 280 533 781 936 Table III: Efficient cloning over a broad range of DNA concentrations Page 4 Headline/Product Name In-Fusion ™ Universal PCR Cloning Kit Compatible with any vector, insert, or restriction site Figure 3. Universal primer . . .
. . .  Creator Acceptor Vectors (Table II). The transfer takes about 30 min and is accomplished in one step by incubating the appropriate vectors with Cre recombinase. There is no need to search for a new restriction site suitable for cloning . The reaction is rapid and error-free since the insert is not amplified but simply moved from one vector to the other. *For more information about these vectors, please see the related vector information page  . . .
. . .  clone and characterize PCR fragments. PCR fragments cloned into either pDNR-Dual or pDNR-CMV with the In-Fusion™ system can be transferred to other BD Creator Acceptor Vectors in a 30 min protocol. For details, see our Creator™ Cloning Kits User Manual (PT3460-1). 30-minute protocol Fluorescent Protein Tags Viral Expression Protein Purification Insect Cell Expression Inducible Expression  . . .
. . .  clone and characterize PCR fragments. PCR fragments cloned into either pDNR-Dual or pDNR-CMV with the In-Fusion™ system can be transferred to other BD Creator Acceptor Vectors in a 30 min protocol. For details, see our Creator™ Cloning Kits User Manual (PT3460-1). 30-minute protocol Fluorescent Protein Tags Viral Expression Protein Purification Insect Cell Expression Inducible Expression  . . .
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