Abstract: . . .  applications Use the same In-Fusion cloning protocol with either your own customized vector or our Creator Donor Vectors for maximum versa- tility in your expression experiments. By choosing our Creator Vectors, you have immediate access to a wide range of our expression systems (Figure 4). After cloning a PCR fragment with the In-Fusion method, use the Creator System to transfer the gene from pDNR-Dual or pDNR-CMV to any of our Creator Acceptor Vectors (Table II). The transfer takes about 30 min and is accomplished in one step by incubating the appropriate vectors with Cre recombinase. There is no need to search for a new restriction site suitable for cloning . . . .
. . .  the insert by strand displacement, and then joins the PCR product to the vector. The resulting clone can be used to transform E. coli (Figure 1). Liquid & lyophilized formats In-Fusion reagents are available in liquid for- mat or in a convenient, pre-aliquotted, lyophilized microtube format. The lyophi- lized format is ready-to-use and only requires the addition of your PCR product and lin- earized vector prior to incubation (Figure 2). No ligase, no restriction enzymes, no phosphatase treatment Figure 2. The In-Fusion™ PCR Cloning Kits make gene cloning quick and easy. In-Fusion reagents are provided in liquid or lyophilized formats. The lyophilized . . .
. . .  Prokaryotic Expression Vectors pLP-PROTet-6xHN High-level, tetracycline-inducible bacterial expression Constitutive Baculovirus Expression Vector pLP-BacPAK9 Constitutive baculoviral expression construct pLP-BacPAK9-6xHN Constitutive baculoviral expression construct with 6xHN tags Creator™ Donor Vector or your vector Cm r SacB loxP loxP Gene Page 5 Clontech Laboratories, Inc. 1290 Terra Bella Ave. Mountain View, CA 94043 USA www.clontech.com BR5Z1348 IN (639904) Asia Pacific Takara Bio Inc. Tel: 81.77.543.7247 Fax: 81.77.543.9254 . . .
. . .  the In-Fusion method, use the Creator System to transfer the gene from pDNR-Dual or pDNR-CMV to any of our Creator Acceptor Vectors (Table II). The transfer takes about 30 min and is accomplished in one step by incubating the appropriate vectors with Cre recombinase. There is no need to search for a new restriction site suitable for cloning . The reaction is rapid and error-free since the insert is not amplified but simply moved from one vector to the other. *For more information about these vectors, please see the related vector information page at www.clontech.com/techinfo. Table II: Creator™ Acceptor Vectors* Matchmaker™ Vectors pLP-GADT7, . . .
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